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And though those were farewells for a few months, and though we are past the days enumerated on that construction paper calendar, I do hope and believe I have not said goodbye to the Five Week Adventure.
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OHT was administered to all rings after 4 days of sprouting, and microvessels were enumerated 4 days later.
The inoculated skim milk broth was held at 6°C for 21 days and bacterial numbers were enumerated every 7 days by plating in appropriate dilutions on plate count agar (PCA; EMD Millipore, Swedesboro, NJ) using an Autoplate 4000 (Spiral Biotech, Norwood, MA).
The fraction of seeds that germinated within 8 days of sowing was enumerated to determine the germination success.
After 6 days, mature osteoclasts were enumerated under a microscope on the basis of the number of nuclei (more than three nuclei) and the tartrate-resistant acid phosphatase (TRAP) activity (Sigma).
B. pertussis-vaccinated, -previously infected, or naïve mice were gentamicin treated to clear any remnant bacteria and then challenged with a gentamicin resistant strain of B. bronchiseptica and the bacterial load was enumerated on Days 3, 7, 10 and 14 post-challenge.
Cells secreting vaccine-specific Ig were enumerated 6 days after vaccination.
Transformed colonies in selected areas were enumerated 10 days after infection.
For cell growth experiments, cells in 5 random fields of view (100× magnification) were enumerated on days 2, 4, and 6 post-infection of rAd/eGFP and rAd/dnStat3.
C57BL/6 mice were infected intranasally under inhalation anesthetic with 100 pfu influenza (H17) and, 2 or 6 months later, injected intraperitoneally with PBS or 2 × 10 pfu rVV expressing OVA (rVV-OVA 36) or rVV-NP 37. Viral load in ovaries was enumerated 5 days later 8, 38.
Colonies formed after a seven-day incubation and were enumerated under a fluorescent microscope before being fixed and further stained with trypan blue (Fig. 1A).
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CEO of Professional Science Editing for Scientists @ prosciediting.com