24 h after transfection, cells were screened by 300 μg/mL hygromycin for two days, and the survival cells were cultured continuously in medium with 2 μg/mL puromycin for ~5 days, during which period, medium was replaced every day to remove the dead cells.
The Ludox dispersion was dialyzed for 5 days against DI water (water changed 3 times per day) to remove remaining salt.
And so the tenants spend their days meeting with the receiver, celebrating minor victories — their lawsuit over Dawnay Day to remove fees from their rent bills — and fearing the future.
We work every day to remove the stigma of it".
Mr. Rayman's work includes cleaning the smoke generators every day to remove accumulated tar.
If possible, vacuum your carpets every day to remove surface dirt and dust.
You can use them throughout the day to remove excess oil without the build up of powder.
This wedding is not just a marriage but a day to remove some sadness that is in our hearts".
The mob eventually settled on a concrete demand, giving editor Bailey until 10 a.m. next day to remove his printing press from the city or have it destroyed.
Junior officers were ordered to rewrite their statements from the day, to remove criticism of the police's operation and emphasise misconduct by supporters.
The parliament voted on the same day to remove him from office, and then the following day Oleksandr Turchynov became acting president.
