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Plasma samples were stored at −80 °C until the day of the analysis.
Samples GC-MS-SPME and EN were prepared on the first day of the analysis taking this day like 0 time.
On the day of the analysis, cells were trypsynized, washed 3 times in ice-cold PBS and resuspended in 1 ml of PBS.
We included all adult patients with a length of ICU stay ≥ 24 hours who were treated in the selected ICU on the day of the analysis.
Carrageenan-gelatin cryogels synthesized with glutaraldehyde as a crosslinker showed a gradual increase till 5th day of the analysis after which a drop was observed.
All the samples, for microbiological analysis, CG-MS-SPME and EN were prepared on the first day of the analysis, taking this day as zero-time.
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Table 4 Dummy variable model of the day-of-the-week effect, subperiod analysis VW EW 1 2 3 4 5 6 7 8 9 10 Panel A: 1953-1970 Mon.
Table 2 Dummy-variable model of the day-of-the-week effect, full-period analysis 1953-2006 VW EW 1 2 3 4 5 6 7 8 9 10 Mon.
To determine the day-to-day repeatability of the analysis, the samples were analyzed by capillary electrophoresis for three consecutive days, performed as sequence of five runs each day.
Unfortunately we did not track the time of the home visit so could not control for time of day in the analysis.
Spiked extracts from S. cerevisiae were analyzed in triplicate during the same day to investigate the intra-day precision of the analysis and is presented as coefficient of variation (CV%).
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