On day 30, testes of Hsp90αflox/flox/CreERT2 but not Hsp90αflox/+/CreERT2 mice showed atrophy, diminishing to a size similar to that of Hsp90α KO testes (Fig. 2D, day 30).
Staged testes (day of birth = 0 d.p.p).
A comparison of significantly regulated genes in 5- and 7-day-old testes of WT and Dazl KO mice with results from the present study showed that just two genes were significantly regulated in both experiments, namely Ovol1 and Nfx2 (Maratou et al., 2004).
In order to discriminate between spermatogonia and spermatocytes, we further performed immunohistochemistry in mirror sections of 8 week and day 17 testes with antibodies specific to synaptonemal complex protein 3 (SCP3) that is expressed in spermatocyte but not in spermatogonia.
Testicular protein extracts were prepared by homogenization of testes from 3-month- or 25-day-old mice in SDS PAGE buffer.
By H&E staining, there was only a marginal reduction of germ cell development in Hsp90αKO day 17 testes; however, developmental arrest became apparent with progressive age (8 weeks) (Fig. 1D, left panel).
We counted the number of testis ova on histological sections in non-irradiated wt Hd-rR and p53-deficient medaka testes (n=3), and γ-ray-irradiated p53-deficient medaka testes at 1 day, 7 days, 14 days and 28 days after irradiation (all time points, n=3), followed by dividing each total area of testes calculated by Image J software (NIH, Bethesda, MD, USA).
While fertile women produce a single egg a month on a predictable cycle, men produce tens of millions of sperm every day in the testes.
Male birds have a pair of testes, however.
Formation of androstanediol was normal in testes from mice deficient in steroid 5α-reductase 2 but absent in testes from mice deficient in steroid 5α-reductase 1, indicating that isoenzyme 2 is not expressed in day 24 26 testes.
