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The effect of different incubation periods on α-Gal production using basal fermentation medium is shown in Figure 2. The optimum production was obtained at the fifth and sixth day of incubation period; maximum activity was shown by WF-3 fungal strain into the culture filtrate (213.63 U/mL) in media containing GG + GAL followed by media containing SM (170.36 U/mL) and others.
The precise timing of the onset or offset were defined as at least three consecutive 10-min bins each with activity reaching at least 10% of the maximum activity per bin during this day.
Software analysis of zymograms indicates maximum activity of the hepatic G6PD levels in day-14 NDEA-treated animals.
Unlike CCA1 and LHY, which were shown to have similar activity at different time of day in ethanol-inducible lines (Knowles et al., 2008), we have found that RVE8 activity is gated with maximum activity in the afternoon.
Another study reported that PGase and Xylase of T. virens, exhibited their maximum activity at day 4 (132 and 75 units/g solid, respectively) [ 31].
The maximum activity of Mro APO was detected on day 24 of cultivation, i.e. 3 to 4 days after the pH had started to increase again reaching a maximum value of 7.5 on day 25.
Maximum activity of each protease was defined as 100%.
It exhibited maximum activity at temperature of 70°C.
However, the endoglucanase activity in the presence of Gl was maintained at a low level, and its maximum activity was only 0.87±0.06 U ml-1 on the 3rd day.
Results are expressed as a percentage of maximum luciferase activity.
Maximum activity was observed at pH 5.0.
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