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Eggs were collected within one day of laying, typically at the 25-30 somite pair stage.
We achieved this by removing up to the first ten eggs on the day of laying while simultaneously monitoring incubation during the entire laying phase.
The nests were visited on the day of laying the third egg, and the eggs were marked with small dots (non-toxic permanent marker).
All females laid one egg per day between 0500 and 1100 and eggs were numbered sequentially on the day of laying.
At 37 of these nests we permanently collected the second and tenth eggs on the day of laying as part of another study (19 control and 18 experimental nests).
The experimental treatment consisted of collecting the first ten laid eggs, each on the day of laying, such that eggs were never present in the nest at night, when females incubate, for the first 10 days of laying.
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Females mated to Jhe or clt mutant males laid equivalent numbers of eggs regardless of the mating trial and day of egg laying (ANOVA, genotype p > 0.05, trial p > 0.05, day p > 0.05), and neither Jhe nor clt mutant females had detectable fertility defects.
This gives a relationship where the proportion of eggs laid increases from 0 on day 0 and converges to an asymptote of 1 on the final day of egg laying.
The Ukrainian foreign ministry released a furious statement on Sunday accusing Russia of laying down unacceptable conditions to a neighbouring country.
Congressman Adam Schiff, the Democratic ranking member of the House Intelligence Committee, did an impressive job in Monday's hearing of laying out a strong circumstantial case for collusion between Russian operatives and members of Donald Trump's presidential campaign.
We also measured the significance of genotype, mating bout and day of egg laying on the male's fecundity (Univariate ANOVA and Tukey's post-hoc analysis).
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