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For mated flies, virgin females and males were aged for 5 days separately from the day of emergence.
Sex and day of emergence were recorded for adults.
Bees were marked with individually numbered tags within 1 day of emergence from pupae.
Mean daily temperatures taken from hourly averages were assessed prior to the emergence period (May 15 to the first day of emergence) and during the flight season (first day of emergence to August 1).
Plants for analysis were selected in phases of stem (32nd day of emergence), budding (40th day), and flowering (50th day).
Both females and males were collected on the day of emergence to ensure mating and were kept in vials with access to honey and water.
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Two plants were retained in each pot after 10 days of emergence, and 20-day-old plants were used for the experiment.
All these parameters were measured with the fifth rosette leaves, which were harvested at specified days counted from the day of leaf emergence.
We designed four experimental schedules with different combinations of short-day and long-day conditions before and after adult emergence and injected dsRNAs or saline into females on the day of adult emergence.
day of wave emergence did not differ between the control and estradiol alone groups, but tended to be later in the estradiol plus progesterone group Day 4.0 ± 0.7, Day 3.5 ± 0.3, and Day 5.2 ± 0.2, respectively; P = 0.06).
The day of wave emergence was more variable (P < 0.05) and tended to be later (P = 0.10) in the control group compared to the ablation group (Day 2.5 ± 0.8 versus Day 1.4 ± 0.2).
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