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A predose urine sample was collected prior to the administration of the oral dose, and 10 subsequent urine samples were collected, one for each of the 10 days after the day of dosing.
11 subjects were enrolled with 3 participants being withdrawn after the first day of dosing.
Consistent with a reduction of cortical mGlu5 receptor density and phosphorylation, CDPPB (30 mg/kg) significantly affected sleep architecture as determined by cortical EEG at day one however by the seventh day of dosing all sleep changes were absent.
The drug solutions were prepared each day of dosing.
Steady state was achieved on the third day of dosing.
Pharmacokinetics (PK) analysis was performed on infected mice on the 20th day of dosing.
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The open field test was performed on day 7 of dosing, the pole test on day 9 of dosing and the rotarod test on day 11 of dosing.
Lastly, there did not appear to be significant differences in drug exposure in dogs dosed between 2.4-2.9 2.4-2.9y day 30 of dosing/ml
Blood smears of allogeneic mice were made for differential counts the last day of drug dosing, the day of death where possible, and on survivors at day 43 post-tumor implantation.
Steady-state conditions were approximately attained on day 5 (96 h) of dosing in the absence of rifampin.
Among the primed subjects who were in study stage 2 (two subjects in F17 and two subjects in F19), the tetravalent rate was 100% on the day of dose 3 and 1 month later.
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