Sentence examples for day of assay from inspiring English sources
Plasma and tissue samples were stored at −80 °C until the day of assay.
DNA samples were stored frozen until the day of assay.
One microlitre of test compound was added to each well on the day of assay initiation.
On the day of assay, test drugs were diluted to 1 mM concentration in complete medium.
All serum samples were stored at −85°C until the day of assay.
All medium and injection reagents were adjusted to pH 7.4 on the day of assay.
The culture was refreshed with 400 μl medium every 4 days until the day of the assay.
On the day of the assay, the samples were diluted in the assay buffer (100 mM HEPES, 10% sucrose and 0.1% CHAPS) in the presence of a final concentration of 50 μM of Ac-Asp-Glu-Val-Asp α- 4-methyl-coumaryl-7-amide) (Peptide Institute Inc., Osaka, Japan; cat. no. 3171-v), 0.0001% NP-40 and 5 μM of DTT.
For the second method, the chemotaxis media plates (10 cm) were poured the day before the assay and were dried for 1 hour at 37°C on the day of the assay.
On the day of the assay, cell viability was tested by trypan blue exclusion dye, and 1 2 × 10 cells/well in assay medium were plated into a 96-well V-bottom plate (BD, Franklin Lakes, NJ).
