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To determine the duration of shedding, infectious virus was identified by seroconversion of rats that had been infected intravenously with 200 µL of 10% suspensions of individual serial fecal samples obtained every other day from rats B76 and B84 during the first 4 weeks postinfection; fecal suspensions were also tested by RT-PCR.
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These cross-comparison studies were performed on seven control urine samples collected at day 7 from rats with no tubular injury as well as the three rats from the 50 mg/kg group from day 7 with the highest (grade 3) level of gentamicin induced tubular injury.
The 14C labeled mannitol released from the drug delivery devices was rapidly cleared (1 day) from the rat urine.
At day 14, spectra from rats exposed to PB demonstrated a shift in the terminal methyl lipoprotein resonance to a lower chemical shift reflecting an increase in high density lipoprotein and decrease in low density lipoprotein as has been reported in PB-exposed Sprague Dawley rats [ 29].
Mixed glial culture was obtained from the postnatal day 1 rats purchased from Japan SLC.
The behavioural tests and the biochemical parameters were assessed as mentioned in group I. Group IV Vehiclee in CCI): Carboxy methyl cellulose (CMC) (0.5% w/v, p.o). was administered for 14 days (starting from day 1st) in rats subjected to CCI.
Every day, starting from day 8, rats were examined for visual signs of disease, defined as macroscopic evidence of increase in paw size.
The day of CFA injection was designated day 0. From day 7, rats were examined daily for the onset of AA by assessing three parameters: body weight, paw swelling, and clinical score.
From day 7, rats were examined daily for the onset of AA by assessing body weight, paw swelling, and clinical score.
One week after rats had adapted to the 12 12-hour 12 12-hourafter surgery, a 24-hour undisturbed baseL De EEG recyclengs were obtafter beginning at dark onsurgerythe 1st recording day in rats from all groups.
The neurons taken from rats 1 day after CFA injection have a more depolarized firing threshold compared with the neurons in naive rats (Fig. 3j).
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