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Intra and inter day precision (coefficient of variation) ranged between 2.41 6.33 and 7.51 8.87 % respectively, while intra and inter day accuracy ranged between 88.98 95.82 and 95.73 102.01%% respectively.
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The inter-day accuracy was determined using the sample recovery on three different days.
The intra- and inter-day accuracy and precision of the analytes were both within acceptable limits.
Inter-day accuracy ranged from 91 to 108% and precision was <2.0%.
Intra- and inter-day accuracy of the method, expressed as relative error, was ≤16%.
The inter- and intra-day accuracy and precision for both TFV and FTC were within ± 20% at the LLOQ and ± 15% at the other QC levels.
The evaluation process of the methodology was executed through determination of analytical parameters, such as selectivity, linearity, accuracy, dynamic linear range, "intra-day" accuracy and limits of detection and quantification.
The intra-day accuracy ranged from 85.8% to 102% for yakuchinone A and from 96.3% to 102% for IS; as well as the inter-day accuracy ranged from 88.1% to 101% for yakuchinone A and 96.6% for IS, respectively.
The corresponding results of intra-day and inter-day accuracy and precision were listed in Table 4.
Both intra-day and inter-day accuracy and precision values of the method are shown in Table 1.
The intra-day precision and inter-day precision (% CV) ranged from 1.73 to 11.38 %, and the intra- day and inter-day accuracy ranged from 97.34 to 103.74%%.
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