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Functional analysis of the 157 upregulated (138 annotated genes) and 323 downregulated probes (301 annotated genes) was performed using DAVID program (david.abcc.ncifcrf.gov).abcc.ncifcrf.gov
Categorization of genes on the list based upon Gene Ontology (GO) assignments were also done using the DAVID program and the outcome showed similar categories to those assigned under the KEGG Pathway (not shown).
For annotation we used the DAVID program [ http://david.abcc.ncifcrf.gov].abcc.ncifcrf.gov]
The DAVID program was used for GO analysis [ 43, 44].
For adjustment by multiple comparisons, the DAVID program used the FDR by the Benjamini procedure.
Functional analysis of the 224 differently expressed genes was performed using DAVID program.
Similar(28)
Functional enrichment analysis based on gene ontology (GO) database was performed by using David 6.7 program (http://david.abcc.ncifcrf.gov/) and protein-protein interactions (PPI) network of DEGs were constructed using STRING database (http://www.string-db.org/).
Expressed proteins in mammary gland tissues of cows were classified by the DAVID online program (http://david.abcc.ncifcrf.gov/).abcc.ncifcrf.gov/
GO enrichment analysis was performed using the DAVID web accessible program [12,13] (http://david.abcc.ncifcrf.gov).abcc.ncifcrf.gov
The lists are provided by the DAVID analysis program [ 17, 19].
All differentially expressed gene sets were then subjected to the DAVID web program [ 35] to identify enriched biological themes and quantify the function categories.
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