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For example, the incorporation of an incorrect nucleotide, or damage caused by mutagenic agents, can be corrected by cutting out a section of the daughter strand and recopying the parental strand.
Daughter strand synthesis.
Mismatch repair also removes erroneous bases from the daughter strand, but does this shortly after daughter strand synthesis [1].
As will be shown below, the continuity of the daughter strand is reestablished following this step.
E. coli utilizes a methyl-directed mechanism to determine the daughter strand and humans use a nick-directed mechanism.
The basic elements of MMR are well conserved across life with a divergence in the mechanism of daughter strand recognition.
Similar(20)
Therefore, both the recF-dependent and the recF recB-independent gap-filling processes repair DNA daughter-strand gaps by transferring DNA from the parental strands into the daughter strands (A103).
Our results suggest that the major role of the Ssb protein in the repair of UV radiation-damaged DNA is to protect single-stranded parental DNA opposite daughter-strand gaps from nuclease attack (A93).
DNA single-strand binding protein plays an important role in the replication of DNA that has been damaged by UV irradiation, and also protects single-stranded parental DNA opposite daughter-strand gaps from nuclease attack, so that postreplication repair can proceed effectively (A93).
We tested this hypothesis by the direct measurement of the repair of DNA daughter-strand gaps.
The recB gene is not required for the repair of DNA daughter-strand gaps, rather it is required for the repair of DNA double-strand breaks that arise at daughter-strand gaps.
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