To test whether the deep split observed between the two chamois was caused by particularly divergent cytochrome b haplotypes of chamois used in this study, we performed the calibrations with all sequences of chamois in databases, including shorter ones (five for R. pyrenaica and six for R. rupicapra), obtaining very similar dates (not shown).
As recommended [ 40], a gene was assumed to be expressed in a tissue significantly if its intensity exceeded the 99th percentile of intensities from the negative controls (Using 90% and 95% as the thresholds gave similar results, date not show).
The transformants showed various antimicrobial activity except H4 (date not shown).
In our other researches, Smaller compound was considered Amino acid derivatives of Monascus pigment homologs (date not shown).
In addition, in the culture extract of the Czapek-Dox medium of A. awamori without LPE, only a single peak was observed after 3 min of elution and no other peaks were found in further elution (date not shown), which drew a conclusion that the fungus could not synthesize those phenolic compounds such as compounds 6, 7, 1', 2' and 5' appearing in the A. awamori-fermented LPE.
Similarly, HIF-1α stabilization was not observed at earlier time points (date not shown).
No significant KA-induced toxic effect was ever detected on glial cells at this concentration (date not shown).
No apparent difference was observed between the Pold1−/− blastocysts (n = 4) and the blastocysts with other genotypes (n = 17) (date not shown).
Histological analysis of swollen thymuses showed a homogenous population of cells with a high nuclear to cytoplasmic ratio and frequent mitoses, typical morphological features of lymphoblastic lymphoma (date not shown).
The phosphorylation level of DCX-T40A was slightly lower (Figure 4D), and other mutations of GST-DCX did not affect phosphorylation (date not shown), indicating that Thr-42 is the major phosphorylation site.
