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In Thoreau's time, the average spring temperature was 42 degrees and the average date of first flowering of the 32 species in the study was May 15.
We checked all plants daily and recorded germination and date of first flowering.
Flowering time was measured from the date of germination to the date of first flowering.
Before the date of first flowering, flats were haphazardly moved about daily in the greenhouse to minimize spatial effects.
To ensure that harvests were completed at a uniform developmental stage, each plant was harvest at the designated number of days after the date of first flowering.
The date of first flowering of individual plants from six populations across the altitudinal range of T. speciosissima (70, 180, 200, 410, 760, 980 m) was recorded during previous site visits (n= 256).
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Date of first square (DFS), date of first flower (DFF), date of first open boll (DFOB), number of node first sympodial branch (NNFSB), and harvested rate before frost (HRBF) as earliness traits of six parents, 15 F1 hybrids and 15 F2 progenies were investigated from 2005 to 2008.
We obtain striking evidence for divergent natural selection on date of first flower production at these two sites.
We recorded date of first flower twice a week beginning on 1 July and ending on 4 September.
For each plant, we recorded seven measures of plant growth, size, or fitness: leaf number at six weeks, date of first flower, number of fruits, fruit weight, dry shoot weight, dry root weight, and number of primary branches.
First, whereas overall there was little variation in flowering time (as measured by days from germination to date of first flower), the neoautotetraploid lines of M. guttatus (IM-4x) (Table S4 and Figure 4) flowered more than 8 days later than all other lines.
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