For the remaining 45 substances, full datasets were not available and it is recommended that in the first instance, attempts are made to fill these data gaps.
Comparisons between two groups were analyzed using Student's t test or the Mann–Whitney U test for experiments in which the datasets were not normally distributed (GraphPad Prism 5.0 software; GraphPad Software, La Jolla, CA, USA).
The GPWv4 datasets were resampled to the spatial resolution of the WorlpPop datasets and used only for countries for which the WorldPop datasets were not available (Supplementary Table 1).
The three datasets were not combined to a single data set for two reasons: First, comparing the results from three different countries constitutes a more conservative approach, increasing the probability of finding genes truly associated with invasive disease, rather than associated with lineages more prevalent in certain datasets.
The molecules in all the datasets were not optimized before performing the EEM parameterization.
We did not attempt to do so since the conditions for pooling the datasets were not optimal.
The smaller HDRA panel size explains why the GBS data were used as priority and why the two datasets were not merged.
With the changing health care landscape and new electronic health record requirements, medical record registries will help validate existing systems, clarify the magnitude of the problem, and can provide critical clinical information that existing datasets were not designed to collect.
The small numerical differences found for the test datasets were not relevant for practical purposes, with the exception of the hockey stick model, where several different starting parameters have to be used in order to find the best fit.
Although the phylogenetic trees of the two datasets were not perfectly congruent with each other, especially in the deeper nodes, several well supported subclades were highly consistent between the two trees (Figure 6).
An ONS spokesman told the BBC the past figures, based on surveys at ports of entry to the UK, could not be updated based on the Census statistics as the two datasets were not comparable.
