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Four publicly available GEO Datasets were further analyzed, and the expression data of the genes of interest (GOIs) were compared to those of the present study.
Datasets were further analyzed by the microarray Database system provided by the Center for Information Technology at the NIH.
The two array datasets were further analyzed using K-means clustering (KMC) for genes whose expression pattern was correlated with the induction of fruit abscission (Additional file 3, Table S2).
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After discovering the possibly damaged variants found in SIFT and Polyphen2, the consensus of both datasets was further analyzed in order to find the most probable impact of these deleterious variants in terms of drug targeting, transport, and metabolism.
Only those genes showing significant differences in this relatively large dataset were further analyzed for specific complications.
In addition, the significantly up-regulated genes from the differentially expressed genes dataset were further analyzed by investigating the corresponding GO biological processes.
Since the Anova approach is not ideal for the analysis of several simultaneous measurements, the dataset was further analyzed using multivariate analysis.
Since PCA could not distinguish between sample batches, disease severity defined by either FVC or DLCO, or familial or sporadic cases, the filtered dataset was further analyzed in aggregate.
In the study, the complete genome of Mycobacterium tuberculosis H37Rv was blasted against DEG to identify essential genes and the resulting dataset was further analyzed for similarity search against human genome to identify genes which are not similar with human to avoid host toxicity.
First, experimental samples were analyzed and conclusions were formulated; and second, experimental sets were combined with publicly available microarray datasets and were further analyzed in search of common DE genes.
Phylogenetic relationships were further analyzed for the dataset of haplotypes of the combined sequence aligned with sequences of Capra hircus (AF533441), Ovis aries (NC_001941) and Bos taurus (NC_001567) as outgroups.
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CEO of Professional Science Editing for Scientists @ prosciediting.com