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The analysis of real datasets from laboratory studies and from real size buildings show that one can reach a root mean square error (RMSE) on strength of about 4 MPa.
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For this analysis we selected datasets from one laboratory using similar methods across tissues (GSE9904, GSE9907, GSE9908, GSE9895) [ 12].
In addition, the method gives consistent results across datasets from disparate laboratories.
Datasets from different laboratories may then be easily concatenated for the purpose of meta-analyses.
Thus, with our analysis, we were able to achieve comparability of microarray datasets from different laboratories addressing different biological questions.
When dealing with heterogenous experimental datasets from different laboratories and experimental settings, efficient data transformation methods are necessary to produce a reasonable level of comparability.
Microarray data are often noisy, and consequently, the reproducibility often is low across datasets from different laboratories for the same disease.
This innovative methodology has recently been used to answer important questions in functional genomics, such as the degree of similarities between microarray datasets from different laboratories or with different experimental models or clinical cohorts.
The 270 genes comprising all 40 signaling pathways (designated as 'pathway signature') are provided in Additional File 6. * By Functional Class Scoring Method In order to demonstrate the reproducibility of the time-course and pathway signatures we utilized two previously published microarray datasets from different laboratories.
Due to the considerable expense of microarray chips and the equipment required, along with the common problem of sufficient sample acquisition, being able to combine and compare datasets from various laboratories and across all microarray generations would be a benefit to the entire biomedical community.
In addition to these datasets, a further total of 80 datasets was obtained from laboratory tests performed on greywacke and agglomerate core samples for this study.
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