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The additional datasets are replicated experiments measuring alteration of the transcriptome through response variation within a single genotype [Development; [33]) and by abiotic stress (Abiotic; [34]].
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The datasets were replicated by randomly sampling from the actual data (sample subject ID with replacement of up to the total number of subjects in the original dataset).
Each dataset was replicated 100 times, and the last consensus tree was decided by majority rule with Consense in the PHYLIP package [ 59].
Furthermore, the predicted probability of LN involvement was negatively associated with age in each subgroup (Figure 3A) in the first dataset, which was replicated in the second dataset (Figure 3B).
In four of these, training dataset findings were replicated in the validation dataset.
30 Given the extensive list of patient and surgical variables considered for inclusion in the model, we wanted to ensure that we minimised the possibility of making a type 1 error (rejecting the null hypothesis when it is true)—for example, the chance that a variable identified as being 'significant' in this dataset may not be replicated in other samples of patients.
In the single-locus analysis of 36,018 SNPs in 129 Rho-related genes, 427 SNPs were significantly associated with CMSS (p < 0.050 and false-positive report probability <0.2) in the discovery dataset, and five SNPs were replicated in the validation dataset.
This has been illustrated previously in this dataset [ 11] and is replicated in Figure 2.
Each simulated dataset and model analysis was replicated ten times for limitIBD of 0.75 and 0.95 and all four window sizes, while in total 52 replicates were considered for limitIBD of 0.55 and all four window sizes.
These observations were replicated in both datasets and could be detected (and replicated) in individual subjects from the HCP.
However, these results need to be replicated using larger datasets collected from different populations.
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