The empirical datasets we use are constrained by the equipment and set-ups that were adopted in the laboratory studies.
We confirm, based on a separate, unbiased dataset, that CNC sequences are constrained, and that human-chicken conserved sequences, which evolved early in the vertebrate lineage have been maintained under selective pressure until our recent past.
One major assumption of the ScHARR model (following the conventions of the Ontario dataset) is that EDSS scores are constrained to remain stable or worsen and improvements are not possible.
Our observations are constrained by the fact that all datasets used in this study were obtained from recombinant progeny clones from a single genetic cross.
But these are constrained, too.
Reconstructed temperatures (Supplementary Dataset) are seasonally unbiased multidecadal average temperatures due to the processes of heat and gas diffusion in the firn layer4,5, and are constrained by borehole temperature profiles4 (Method; Fig. S4).
CIOs are constrained by obsolescence.
However, as this was a secondary analysis of an existing dataset, we were constrained by the food measures recorded in the dataset.
For each in slico simulated dataset, region selections were constrained to not overlap with previously selected regions.
Then, we used the first n principal components (PCs) that explained 95% of the variance to find good representatives of the variation in the dataset, while n was constrained to be smaller than six.
However, the scope of analysis possible with datasets from most HDSSs is constrained by the lack of integration with other administrative data, including those emanating from health facilities.
