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It should, however, be taken into consideration that many structures in our dataset were determined using only a fraction of the actual binding partner, allowing for more flexibility in the protein's termini, which would be restrained in the full protein.
The dataset was originally prepared using the following criteria: all the structures in this dataset were determined using X-ray crystallography with resolution ≤2.0 Å, R-factor ≤0.25 and R-free factor ≤0.25; sequence length should be greater than 60 amino acid residues and without any chain breaks; each two sequences have sequence identity less than 30%.
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The most appropriate model of nucleotide substitution for each dataset was determined using jModelTest v0.1.1 [78].
The overall ML tree topology for each gene and the concatenated dataset was determined using GarliV0.951 [61] with model parameters as estimated by Modeltest.
Significant overrepresentation of particular GO terms in the dataset was determined using the software GeneMerge with corrections for multiple tests [50].
To highlight the differences in transcriptional profile of each tissue of M. galloprovincialis the similarity between the contiguous sequences from each dataset was determined using an all-against-all BLASTN analysis (Table 2).
Variance across the dataset was determined using the Levene test (17 ).
The region overlap between the NKX2-5 and MEIS dataset was determined using Galaxy (Goecks et al., 2010).
The probability of a genotype occurring more than once in the dataset was determined using the formula (2) ∑ x = n G G ! x !
The length of run (number of generations) for each dataset was determined using AWTY graphical system [ 44] to check the convergence of MCMC.
The sufficient number of generations for each dataset was determined using the AWTY graphical system (Nylander et al., 2008) to check for convergence of MCMCMC.
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