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This was because accuracy with kNNI for this dataset was very low overall.
For integrity reasons the NPS did not publish data for PPQ when the total number of individuals in a dataset was very low (n < 30).
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Indeed, the risk for selection bias in this nutritional dataset is very low.
However, the numbers of variable and informative characters of the ITS molecular dataset were very low (27 on ITS1 and 21 on ITS2 for the whole section, i.e. 14 species).
The original dataset was very small.
The N/A rates in most test datasets were very low (<5%, Additional file 1: Table S2).
The haplotypic diversity for this dataset is very high (h = 0.896), but the nucleotide diversity was very low (π = 0.00314).
It was very low key.
Turnout was very low, at about 18%.
His voice was very low, tentative.
Water production was very low.
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