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Lee revisited the subject last year and expanded his dataset to include a "Super Court" of 36 justices and 24 nine-member courts from 1946 to 2016.
Subsampling the dataset to include a single species from either each taxonomic family (n = 28) or each order (n = 15) demonstrated a high degree of phylogenetic independence, with the trend remaining significant in 91% (family) or 86% (order) of the subsampled dataset replicates.
To test the robustness of the topology as well as to resolve the orthology relationships between the members in sponge and other bilaterians and pre-bilaterians, we expanded the dataset to include a few representative sequences from the Trichoplax, Nematostella and sea urchin GPCRs.
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By broadening previous datasets to include a more diverse sample of primate species, we were able to conduct a more expansive test of the relationship between female distribution and male distribution.
We have expanded these datasets to include a greater number of taxa, we have analyzed all of these genes for evidence of systematic biases and we have used improved models of codon evolution.
However, refiltering the dataset to include all variants with an allele frequency of less than 0.01 in the 1000 genomes dataset [Clarke et al., 2012], identified a second mutation, c.2440C>T (p.Ars10489441904894419, allele frequency 0.003), in all five cases.
For this analysis we restricted our dataset to include only the nine A. kamchatica populations for which we had at least three samples.
The third dataset was analyzed to include a geographically critical sample collected near Pinnacles in San Benito County (MVZ 213101), for which only 2 nuclear genes could be sequenced.
In addition, it is important that the number of considered cells (i.e., the network's size) in the datasets is large to include a significant number of faulty cells.
Because admission records from the first year in the dataset (1998-9) were likely to include a mixture of first and subsequent admissions, we excluded 1998-9 data from the analyses.
We selected as datasets to include those that are on a single-channel platform, have survival information and contain more than 81 patients (see 'Robustness analysis' section below).
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