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Combining this subset of sequences with those from the other hosts yielded a final dataset of 2,392 sequences; this dataset had notably fewer sequences from Africa, China, and Southeast Asia than did the original dataset (Technical Appendix Table 2).
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Phylogenetic analyses were performed by using Bayesian and maximum likelihood methods on env and LTR datasets (Technical Appendix).
The RI of the complete dataset, the technical characters and the design characters were all around 0.59.
Finally, in rat GDS1629 dataset one technical replicate has been used for each biological replicate.
This dataset contains technical and biological replication for both studied conditions.
TM calculated the ortholog dataset, provided technical support for database storage, and wrote sections of the manuscript.
To address this issue, we used a dataset on technical and biological replicates of blood gene expression.
We demonstrate the advantages of our method using simulated data as well as an RNA-seq dataset with technical and biological replication for both studied conditions.
We here provide a systematic study of the reliability of expression level estimates from an extended dataset with technical replicates (3×331 million reads).
We here report on a comprehensive systematic study of the reliability of expression level estimates from an extended dataset with technical replicates (3×331 million reads).
The entire microarray dataset and technical information requested by Minimum Information about a Microarray Experiment (MIAME) are available at the Gene Expression Omnibus (GEO) database under accession number GSE16836 (Transcriptional profiling of CD16 + and CD16 - peripheral blood monocytes from healthy individuals).
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