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Although sequencing facilities send these data to their customers on Terabyte-scale hard disc drives, such data volumes are certainly not suitable anymore for upload to data analysis servers or INSDC databases for submission, even not with fast user datagram protocoll (UDP) protocols such as Aspera Connect [ 138].
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Resulting sequences were resubmitted to the relational database and prepared for submission.
While the NCBI peptide database is open for submission of new sequences without external validation, the TEM mutation table is curated by experts in the β-lactamase field.
One of the important data sources of the NCBI protein database is the NCBI nucleotide database which is open for submission of new sequences without further validation; therefore it is growing rapidly, but contains inconsistencies.
This was achieved by searching the journal's database for consecutive submissions in the analysed period 2010 2011.
We are thankful for the help of Patrick Chapman for contributions in tabulating microarray data for database submission.
In addition to annotating the strains, users have an option to contribute their novel variations to the database by entering necessary details for submission on a submit page.
Establish access protocols and address privacy issues such as restrictions for who can input content and approvals of materials for database submission.
For submission to other databases or publication, these data can be exported by the user directly from the database in PDF format.
In total 117 of the draft genome sequences (101 which passed all quality controls and an Additional 16 that had no evidence of chimeric contigs) were considered suitable for submission to public databases (see Additional file 1: Table S1).
We provide a metadata checklist for database submission and publication of UViGs designed to be flexible enough to accommodate technological and methodological changes over time (Table 1 and Supplementary Table 1).
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