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To exclude the possibility of drug resistance mutations impacting on the entropy calculations, epitopes containing amino acid positions known to acquire mutations causing high, intermediate or low level resistance to RT and protease inhibitors (Stanford University Drug Resistance database) were removed from the list of epitopes and all the calculations were repeated.
Those ORFs with a top BLAST hit to a non-methanogen or with no homology to the nr database were removed from the analysis, as were transposase sequences (which are unlikely to represent good vaccine targets), while adhesin-like ORFs are dealt with separately above.
Short sequences that matched only one of the Bet v 1 fragment HMMs provided by the Pfam database were removed from the list.
The sequences matching non-coding RNAs (rRNA, scRNA, snoRNA, snRNA and tRNA) deposited in Rfam 10.1 database were removed from the sequences.
Probes from uncertain chromosomal loci (Chr -random, Chr -randomm, chrE22C19W28_E50C23, chrE64, and W chrE22C19W28_E50C23chrE64tandse) Were removed from the results.
Similar(55)
Identical compounds found in both ACD and MDDR databases were removed from ACD.
Identical sequences, such as those obtained from both Uniref90 and Uniref50 databases, were removed from further analysis.
In order to facilitate certain comparisons of the non- Dhc communities, all reads assigned to Dhc under MG-RAST's phylogenetic profiles for the SEED, Silva LSU, Silva SSU, RDP, and Greengenes databases were removed from the datasets, and a second automated annotation conducted for the " Dhc-subtracted" metagenomes.
Lastly, to avoid classification of non-coding ORFs those specified as 'dubious' (≈ 500) by the Saccharomyces Genome Database (SGD) were removed from each dataset.
Totals of 24, 172 and 97 sequences in SS, RWS and TWS, respectively, showed no affiliation against the UNITE and/or GenBank databases, and were removed from the analyses (Additional file 3A).
Those sequences that could not be found on Swiss-Prot were queried in the computer curated TrEMBL database; otherwise, they were removed from the alignments.
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CEO of Professional Science Editing for Scientists @ prosciediting.com