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For each T. adhaerens protein, the top 50 matches (based on E value) in each database were pooled and ranked according to a comparative sequence similarity match score, Sm (eq. 1): (1) where b is the bitscore of the match, I is the percent identity (%ID), and Q is the percent length of the query that aligned.
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In a second study, clinical data from patients carrying SCN5A mutations leading to Brugada syndrome from the Nantes and Amsterdam databases were pooled (n = 108).
To test this strategy, a mixture of known ESTs (18,490 pea and 17,198 S. sclerotiorum ESTs) from public databases were pooled and parsed; the tBLASTx method successfully separated 90.1% of the artificial EST mix with 99.9% accuracy.
*To assess the potential of the tBLASTx method for the parsing of pea and S. sclerotiorum EST contigs, 17,533 and 18,547 known S. sclerotiorum and pea ESTs from online databases were pooled, the ESTs were then parsed using the tBLASTx method.
The data set to be used for clustering can be owned by a single entity, or in some cases, information from different databases is pooled to enrich the data so that the merged database can improve the clustering effort.
For Cohort 2, a 1 up to 2 ratio was applied to DET-C and GLA-S patients, with the two independent databases being pooled together.
As compared with post-marketing safety studies on healthcare databases, where multiple databases are pooled to increase statistical power, spontaneous reporting databases should not be combined, but analyzed separately to maintain country-specific differences.
Patient-level information from two large healthcare claims databases the Thomson Reuters MarketScan Commercial Claims and Encounters and Medicare Supplemental and Coordination of Benefits Database (MarketScan Database, 2001 2010) and the Intercontinental Marketing Services LifeLink Database (LifeLink Database, 2001 2008)–were pooled for analyses.
Databases therefore were pooled from 4 contemporary trials of primary or rescue PCI (EMERALD, COOL-MI, AMIHOT, and ICE-IT), in which the primary end point was infarct size assessed using technetium-99m sestamibi single-photon emission computed tomographic imaging, measured at the same core laboratory.
Patients from Nantes database with SCN5A-related BrS were pooled with their counterparts from Amsterdam database.
Enrollment in the 2 protocols was completed in September 2013, and data were pooled after database lock in each study.
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