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All digitized data in the database were obtained using this method.
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Fortunately, one bovine genomic DNA contig (AAFC03056271) from the 7.15× bovine genome sequence database was obtained using the human cDNA sequence, and alignment of the human cDNA sequence and the bovine genomic DNA sequence unraveled the genomic organization of the bovine CRHR2 gene, including the promoter region.
Spearman's rank correlation coefficient between selected lncRNAs and other remodeling-related transcripts from Gene database was obtained using the R package WGCNA [ 30].
Access to the database was obtained using DbVisualizer version 7.0.5 (http://www.dbvis.com), a graphical user interface that allows developing and accessing database management system (DBMSs) in different operating systems.
The similarity of these sequences to those in public databases was obtained using Blast analysis (http://www.ncbi.nlm.nih.gov/BLAST/, last accessed January 24 , 2014.
The identification false rate was estimated using a decoy dataset that was constructed by reversing each protein sequence in the human sequence database; no random hits were obtained using the UStags as the identification criteria.
All data were entered into a database, and descriptive statistics were obtained using IBM SPSS statistics version 20.0.0 (IBM Corporation, 2011).
More widespread analyses on the occurrence of severe maternal morbidity in Canada, for instance, were obtained using databases containing information routinely collected in healthcare services [ 25].
The results described here and in the literature (Table 8) were obtained using a database selected and cut out from continuous, multichannel EEG recordings after a visual inspection for artifacts such as muscle activity or eye movement.
Transcription start sites for each gene selected for detailed promoter analysis were obtained using the database of transcription start sites version 6.0 [ 21].
The observations outlined above were obtained using a database derived from a large number of different tissues originating from different donors.
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