Through this approach, the need for data entry into a database is eliminated, which is attractive not only because it reduces the amount of personnel and time dedicated to administering PRMs, but also because it may reduce data entry error.
To reduce the probable germline variants, the single nucleotide polymorphisms (SNPs) that were registered in the dbSNP and in-house Japanese SNP databases were eliminated.
Gene redundancies, due to the use of gene synonyms in source databases, were eliminated by mapping gene symbols to the currently approved HGNC and NCBI identifiers.
Sequence matching non-coding RNAs, including rRNA, tRNA, snRNA, and snoRNA, in the NCBI GenBank (http://www.ncbi.nlm.nih.gov/genbank/), repeats (http://www.girinst.org/repbase/update/index.html) and Rfam (http://www.sanger.ac.uk/software/Rfam) databases were eliminated.
Resulting from this, the mismatch in data that can occur when using two separate databases, is eliminated.
Almost all recruited practices (229/231 = 99%%) generated data into the NHGDoc server database; two were eliminated from the trial due to technical problems.
In our analysis, poly(A/T -tailed ESTs thA/T -tailed in thESTsfSeq mRNA dathatse by blast searchadg were eliminated as known transcripts.
The collected data were kept confidential by the investigators, and patient identifiers were eliminated from the database once the master database had been created.
The specificity of all primers designed was submitted to BLASTn search against NCBI B. napus nr and EST databases and any nonspecific primers were eliminated or redesigned.
Firstly, the sequences matching non-coding RNAs [tRNAs, rRNAs, small nucleolar RNAs (snoRNAs) and small nuclear RNAs (snRNAs)] deposited in the Rfam 10.1 (http://www.sanger.ac.uk/Software/ac.uk/Software/Rfam) and NCBI GeneBank databases (http://www.ncbi.nlm.nih.gov/GenBank/) were eliminated.
