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Sequences that were not in the database were checked by resequencing, and then assigned as new alleles and deposited in the MLST allele database.
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Each name in the sound database is checked by searching for that name via the EoL API.
The reliability of these databases was checked by comparing it to the National Cause of Death Register.
To increase the reliability of the research, databases, journals, and individual papers were checked by a second researcher and updated iteratively during the revision cycle of the paper by the entire team of authors.
All gene annotations were checked by using online tools and databases: Entrez Gene (http://www.ncbi.nlm.nih.gov).nih.gov
Comparisons of shared targets were checked by name in the Gene Ontology database [ 22].
Optimized oligonucleotide sequences were checked by the Basic Local Alignment Search Tool against the GenBank database for in silico specificity.
The database was checked to ensure no duplicates in memberships.
The intein database was checked at New England Biolabs InBase [ 30, 58].
Primer specificities were checked by alignment between cDNAs and by BLAST against NCBI databases.
All three databases were checked for all articles.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com