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Small RNAs that did not map to any miRNAs in miRBase database were analyzed as novel miRNAs using miRDeep2 (developed by ABlife Inc).

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Arabidopsis data were analyzed as follows.

The 776 sequences longer than 3000 bp without hits in the plant database were analyzed further as it is highly unlikely that sequences of this length are comprised of 'nonsense' assemblies.

To this end, all 5'UTR of the different splice variants and the expressed sequence tags (ESTs) as described in the NCBI database were analyzed (Table 5).

Luciferase expression was analyzed as above.

Microsatellite instability (MSI) was analyzed as well.

Amount of tobacco or alcohol consumption was not available through the database, so these data were analyzed as simple dichotomous variables.

From an initial set of 33,030 citations retrieved from multiple databases, a total of 30 articles were analyzed as full reports according to our explicit selection criteria, finally yielding 14 studies eligible for inclusion in our review.

A subset from the KEGG database is analyzed.

The fixed database was analyzed using SPSS11.0.

Database was analyzed using SPSS 9.0 for Windows.

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