Although these methods enable the identification of coherent expression changes, the discovery of new pathways of organizational units, which have no records in the database, is not possible [ 47].
The direct comparison to studies that use the healthy controls in PTB database is not possible, since the authors have applied their methods on part of the healthy persons (12 or 13 from all 14 patients) and have mixed them with cases that do not have separated in time ECGs.
Information was obtained mainly from one source, and cross-checking with other databases was not possible.
Because of the retrospective nature of the current database, it was not possible to clearly define at what time point exactly the patients suffered from the different afflictions characteristic of CGD.
From the available database, it was not possible to differentiate between mild and severe injuries.
With the existing database, it was not possible to identify multiple births during the observational period for a particular mother.
From the database, it was not possible to determine the exact reason for the non-presence of these two LFTs.
While all values of macro nutrients (carbohydrate, protein, fat) could be searched from the database, it was not possible to get all micronutrient values for local food items.
All-cause deaths were age-adjusted by 5 age groups (0 4, 5 24, 25 44, 45 64, and >65 years); because of the structure (aggregate data) of the database, it was not possible to age-adjust other rates.
While several duplicated genes could be mapped to central metabolic pathways from the KEGG database, this was not possible for horizontally transferred genes (Additional file 16 Figure S 6 and Additional file 17: Figure S 7).
