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This score database was generated using a set of state-of-the-art baseline classifiers along with template-based approaches.
The D. melanogaster phenotype database was generated using the Flybase QueryBuilder.
Similarly, the C. elegans phenotype database was generated using the Caltech server based on Wormbase release 177 [23].
A clinical database was generated using patient information from Manitoba.
The initial ChlamyCyc database was generated using the PathoLogic Pathway Prediction module of Pathway Tools version 11.5.
Second, the drug activity database was generated using a single assay end point, that is, short-term growth inhibition and cytotoxicity.
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The ANN database is generated using USEPA NAPL-Simulator.
Protein alignments of translated cassette-encoded genes as well as intI sequences and their homologs from the NCBI database were generated using ClustalW [47] and manually edited to remove ambiguous positions.
The web interface of the EuroDia database is generated using a combination of JavaScript and PHP scripts that form the GEDAI framework.
Importantly, the gene expression profiles in the database are generated using multiple types of primary cells, such as liver, brain, and skin.
Data presented in the demonstration databases were generated using an Applied Biosystems 7500 instrument (normal ramping), Qiagen QuantiTect in a 10 µl reaction volume containing 500 nM of primers, in 96 well white plates (ABgene) sealed with MicroAmp film (Applied Biosystems), and amplified using a cycling regime of 15 min activation at 95°C, followed by 50 cycles of 95°C −10 s, 65°C −120 s.
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