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A reverse database was appended and concurrently searched.
Discrepancies were verified with patients' written notes if necessary and the database was appended accordingly.
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The genic repeats have been further hyperlinked with their corresponding locus id, and the database is appended with primer3 plus for primer designing of selected repeats with left and right flanking sequences up to 250 bp.
Furthermore, randomized versions of the applied databases were appended to the original databases using the decoy perl script (Matrix Science, Boston, USA) downloadable at.
The herbarium record database for each species was appended to the "habitat" variable database, which was exported in tabular format for statistical analyses.
Proteins were identified against the B. pumilus entries from the RefSeq protein database available at NCBI, which was appended to an existing bacterial database.
The sequence of PKN1 3 was appended to the database, and peptides were searched directly against that protein sequence.
The data were searched against the Saccharomyces Genome Database (downloaded 02/03/2011; www.yeastgenome.org) that was appended with protein sequences from the common Repository of Adventitious Proteins or cRAP (www.thegpm.org/crap/).org/crap/
A decoy database, in which the sequences had been reversed, was appended to reduce false positive identification.
To each database a set of common laboratory contaminant proteins (73 entries) was appended.
This footnote was appended on 26 July 2011.
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