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Position-specific-iterated BLAST (PSI-BLAST) iteratively searches a protein sequence database, using the matches in round i to construct a position-specific score matrix (PSSM) for searching the database in round i + 1.
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RESULTS: Sixty-six patients were selected from our database using the matched-pair method.
After identifying a region of high divergence, we extracted the sequence from both the low and high divergence clades and searched for transcription factor binding sites by comparing the sequences to insect matrices within the TRANSFAC database using the Match 1.0 webserver (BioBase).
Transcription factor binding sites were identified by searching the promoter sequences against the vertebrate matrix data set of the Transfac 9.0 database, using the software Match (www.gene-regulation.com).
E-pharamcophore, vROCS, and glide SP docking were performed on Asinex datasets in a step by step process.To select the best scoring molecules from the database of Asinex we first screened the database using the e-pharmacophore matching at least 4 features out of five present in the Pharmacophore model.
The MGF file was searched against the S39006 proteome database using the Mascot search algorithm to yield matches of MS/MS spectra to peptides.
Thereafter, all four data streams are collocated into a navigated 1-min resolution match-up database using the UTC time coordinate.
629,493 yielded the same CHI number on matching to the CHI database using the fields in our linkage methodology.
The peptide masses obtained were matched to peptide mass databases using the ProFound and MASCOT software.
We use funneled version of the database [21], used the match and non-match sets provided by the database and followed 'Strict LFW' protocol.
When applicable, annotations were transferred from those of M. tuberculosis orthologs in the TubercuList/Mycobrowser database, using BLAST matches of > 90% protein sequence identity, an alignable region of >80% of the shortest protein length in pairwise comparisons and visual inspection of the gene synteny.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com