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Using the open source search tool OMSSA (version 2.0.0) [25] the spectra were matched against an N. vitripennis sequence database retrieved from NCBI (http://www.ncbi.nlm.nih.gov/) containing additional trypsin and keratin sequences.
Once the SAGE library of interest is selected, such as GSM673.lib (indicated by the black background on the left side of Figure 1C), the detailed information for this SAGE library is provided (the right side of Figure 1C) via mining of the local database retrieved from GEO of NCBI (GSM673 is renamed GSM383828 by GEO; http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi acc=GSM383828).nih.gov/geo/query/acc.cgi acc=GSM383828
To get the human homologous genes the refGene database retrieved from the UCSC Genome Browser database was used [ 29, 32].
These nonredundant target sequences were further compared against the entire nt database retrieved from NCBI in July 2007.
The gene network co-expression patterns were compared with a manually compiled protein-protein interactions (PPI) database retrieved from the Human Protein Reference Database HPRDD) [ 32, 33].
All these repeats in the custom library were compared to a Viridiplantae TE database retrieved from Repbase (http://www.girinst.org/repbase/index.html, update 20130422) (15) and a Plant Repeat Database (http://plantrepeats.plantbiology.msu.edu/index.html) (16), using tBLASTx and BLASTn.
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They were blasted against GO database (http://www.geneontology.org/), Swissprot, Ref_protein and Refseq_RNA databases that retrieved from NCBI database using Blastn or Blastx algorithm with an E-value threshold of E−5.
The NCI database was retrieved from the NCI download site (dtp.nci.nih.gov/docs/3d_database/Structural_information/structural_data.html) and converted into a 3D database using Catalyst 4.11 software (www.accelrys.com).
Twenty one IFABP protein sequences, sixteen of which from fish databases, were retrieved from the NCBI database (http://mirrors.vbi.vt.edu/mirrors/ftp.ncbi.nih.gov/blast/db/).nih.gov/blast/db/
In addition, data about the usage of certain tags in the OSM database are retrieved from Tag History (http://taghistory.raifer.tech).raifer.tech
The DEG 6.8 database was retrieved from http://www.tubic.tju.edu.cn/deg/.
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