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He started by creating a database of elements and references, cataloguing plants, flowers, and old botanical illustrations.
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The web-based CENSOR tool [ 108] was used to mask sequences and identify transposable elements by comparison to the Repbase database of repeat elements [ 66].
Using the major database of repetitive elements, Repbase [ 32], we list 12 specific repetitive elements for the C. floridanus genome and we give a summary of these elements in Table 1.
Representative web-databases of transposable elements in the human genome have been reported: a database of Alu elements incorporated within protein-coding gene [ 2], an HERV expression and structure analysis system [ 3] and a system for extrapolating functional annotation to the prediction of active LINE-1 elements [ 23].
We examined the potential that transcripts may include transposable elements by performing a blastn (e−10) analysis using a database of transposable elements developed from randomly sampled Ipomoea purpurea genome sequence and the program Assisted Automated Assembler of Repeat Families (i.e., AAARF; Debarry et al. 2008; R. S. Baucom and J. Bennetzen, unpublished data).
In that study, SINEs were identified using RepeatMasker [ 18], a program that identifies putative repeat elements in genomic sequences by comparison to a pre-compiled database of repetitive elements.
Transposon elements were annotated using TBLASTX searches (E-value ≤ 10−5) against the database of transposon elements, RepBase (Jurka et al., 2005).
One of these genes (PP8) had a significant sequence identity to a gypsy element when using the tBLASTx algorithm to search the Repbase (a database of transposable elements; Jurka et al. 2005).
Finally, the annotated sequences were compared with the RepBase database of transposable elements (TE) [ 54].
A database of EhSINE1 elements was built based on the results generated by Huntley et al.[ 30].
The most widely used repeat masking tool, RepeatMasker [ 12], relies on an external database of repetitive elements.
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