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Donohue says that the team had no knowledge of the database limit, and there was nothing that would have alerted them to the fact.
The PERL/C implementation described here has a database limit of 2 billion sequences, but this limit can be lifted by changing constants within the source code.
Specifically, the Advanced BLAST function was used, set to search only the S. mansoni sequences in the GSS database
Specifically, the Advanced BLAST function was used, set to search only the S. mansoni sequences in the GSS database
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Additionally, the lack of a centralized toxicity database limits comparison between research results.
Furthermore, specificity tests will be performed against reduced database limiting computational resources needs.
Spectra were searched in MASCOT (http://www.matrixscience.com/search_form_select.html) against the NCBI non-redundant database limited to E. coli.
Database similarity searches with the derived sequence stretches were performed using the short sequence blast algorithm against the non-redundant protein database limited to green plants.
Finally, non-CLI symptomatic or asymptomatic PAD status was also not consistently reported in our database, limiting possible inferences on such patients.
EST abundance for each Glo-3 mRNA was measured using MegaBlast against GenBank "non-mouse and non-human EST database limited to Triticum (total of 1,106,281 sequences, 22.03.09).
BLASTN was used for similarity searches between CRISPR sequences and existing sequences in the DDBJ [ 122] database limited to viruses (ddbjvrl and ddbjphg) or bacteria (ddbjbct).
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