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At first, a complete database is submitted to random data extractions and then the proposed method is applied.
The information in that database is submitted by each institution, which may or may not be recognized by CHEA.
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Two 16S rRNA sequences with poor matches to the database were submitted to NCBI.
The sequence types not present in the database were submitted to the website and assigned new ST types.
Sequences of any alleles that were not in the database were submitted to the curator and a new allele number obtained.
Two separate transcriptomic datasets were generated and raw sequences from each database were submitted to the Sequence Read Archive [SRA: SRR350710.3 and SRR351237.2].
Each random database was submitted to the same all-versus-all alignment approach used for the real data, and the distribution of alignment scores considered the mean ± SD.
Refseq accession numbers for those genes predicted to contain NR2E1 binding sites in the oPOSSUM database were submitted to the DAVID service (http://david.abcc.ncifcrf.gov/summary.jsp) for GO term annotation enrichment analysis [ 50, 51].
A total of 1686 pea sequences (290 sequences from C library, 300 sequences from S library and 1086 ESTs from the N library) together with 500 pea sequences randomly selected from GenBank pea protein database were submitted for probe design using the open source CombiMatrix probe design system.
Gene-expression strength information in the source databases is submitted by the authors or extracted from the literature by database curators.
DHPLC Novel SNPs not in dbSNP or HapMap databases were submitted to dbSNP.
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