Sentence examples for database forward from inspiring English sources

Columns indicate marker name, EST or Contig information in the CGPDB database, forward and reverse primers, annealing temperature (Ta), magnesium concentration in PCR reaction, and size of amplicon.

Primers to amplify γ-gliadin genes from genomic DNA using PCR were designed on the conserved sequences at the 5' and 3' end of the coding region of γ-gliadin gene sequences obtained from the EMBL database (forward primer, γ1F: 5'-atgaagaccttactcatcc-3', and reverse γ11R: 5'-ggacaWagacRttgcacatg-3').

Primers to amplify α-gliadin genes from genomic DNA using PCR were designed on the conserved sequences at the 5' and 3' end of the coding region of the α-gliadin gene sequences obtained from the public database (forward primer, 1F: 5'-ATG AAG ACC TTT CTC ATC C-3', and reverse primer, 5R: 5'-GTT AGT ACC GAA GAT GAT-3').

Primers were designed from protein sequences available in the public database (enolase forward: 5' TCCCGYGGKAAYCCMACHGTKGAGGT 3', reverse: 5' GGKAGRATCATRAAYTCYTGCATRGC 3'; pyruvate kinase forward: 5' TTCTCYCAYGGMWCSYACGAGTAYCA 3', reverse: 5' CGRAYRAAMGARGCRAASAYCATGTC 3').

Based on the feature of the stem-loop structure, a command line BLAST was performed to align each unique miRNA sequence with the sequences of the transcriptomic database, aligning forward and reverse strands separately by setting the parameter –S (1 for forward, 2 for reverse alignment).

Both the Tories and the Liberal Democrats opposed plans for a DNA database put forward under Labour, partly due to concerns that it could affect people's civil liberties.

Also, these modifications will also be incorporated into Foursquare's venue database going forward.

Data processing and databank searching were performed with Mascot software (Matrix Science, Beachwood, OH) and a database containing forward and decoy sequences (RSUE database) that was constructed using the USA300_FPR3757 sequence.

A database containing forward and reverse entries of all rat proteins listed in the UniProt database (version 12th Dez 2013) was utilized.

MS/MS-data were exported directly from Progenesis LC MS in mgf format and searched against a decoy database the forward and reverse sequences of the predicted proteome from S. cerevisae (SGD, download date: 15/6/2012, total of 13,590 entries) using MASCOT (version 2.4.0).

To cross-check the primer-designing potential of ILP markers and validate the results of the PIP database, the forward and reverse primers designed for the ILP markers were BLAST-searched against latest released foxtail millet pseudomolecules of nine chromosomes (http://www.phytozome.net).net