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The AGF captures player actions and data parameter values in a database, expressed as eXtensible Markup Language (XML) elements.
Functional class distribution of the 213 genes found in our analysis compared to the general distribution in the full COG database (expressed as percentages).
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For initial analysis, reporter plasmids were transfected into the Y79 retinoblastoma cell line, which based on the NCBI EST database expresses RdCVF, and into the non-retinal cell line HEK 293, which was derived from adenoviral DNA transformed human embryonic kidney cells [31].
The sequence of this signal sequence was employed as query to search for matches in the GenBank non-redundant nucleotide sequence database and expressed sequence tags (ESTs) database for all organisms using BLASTN program in National Center for Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLAST) [ 71].
The models yielded 1221 additional sequences from the nr-database at NCBI and various genome projects (DOE Joint Genome Institute, Baylor College of Medicinee, J. Craig Venter Institute and Broad Institute) and several EST databases (NCBI Expressed Sequence Tags database, Fungal Genomics Project, and Taxonomical Broad EST Database) [ 24].
To ensure robustness of the database, only mRNA sequences or complete coding cDNA sequences (CDS) were retrieved from the NCBI database, excluding expressed sequence tags (ESTs), sequence tagged sites (STS), genome sequence survey (GSS), working drafts and patent sequences.
We have developed a foundational database of expressed sequences and collection of plasmid clones that comprise even low-expressed genes such as those encoding transcription factors.
The entire fingerprint database is expressed as. mathrm{D}=left[{R}_1,,dots,,dots, {R}_wright] (3)whereW is the maximum number of the reference points in the radio map.
After assembling a database of expressed sequence tags--short DNA sequences that can be used to identify a gene expressed in a cell at a given time--Reppert and his colleagues located two families of cryptochromes in the monarch.
CKT provided access to the Iowa State University database of expressed pig sequences (http://www.anexdb.org).
The technique of 454 RNA sequencing was employed to produce a database of expressed genes of I. indigotica.
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