In three of the microorganisms (Collimonas arenae, Achromobacter xylosoxidans, V. paradoxus), the mRNA of the 3-HP catabolic genes was not amplified at all, which suggested that the degenerate primer sequences, designed based on NCBI database, did not match the original gene sequence (Additional file 3: Table S3).
According to previous studies [ 2, 28, 29], simplified RIFLE classification was used only with the glomerular filtration rate (GFR) criterion for classification because the eight-hourly urine volumes in our database did not match the 6- or 12-hourly urine output criterion in the RIFLE classification.
For example, the identifier "floxuridine" occurs in ChEBI and in ChEBML, but the corresponding structures in these two databases do not match, and thus the identifier is ambiguous.
Two predicted glycosyltransferases, Gtr31 and Gtr30, belong to the GT4 family of retaining glycosyltransferases in the Carbohydrate Active enZYmes (CAZy) database (Lombard et al. 2014), whereas Gtr59 does not match any structure in the CAZy database and represents a novel family.
The DNA evidence does not match anyone in the F.B.I. database.
It does not match any mammalian sequences currently available on online databases.
We subsequently removed all sequences within our database that did not match one of these haplotypes.
Forty percent of the assembled contigs in the E. lineata transcriptome produced BLAST hits to sequences in NCBI's non-redundant (NR) protein database, while 60% did not match any protein sequences in the database.
The unidentified man's fingerprints were not in any law enforcement database and he did not match any missing person reports.
This eliminated the shortest sequence tags, those that matched with multiple genes or genes with multiple differing transcripts, those that matched genes not yet contained in the GenBank non-redundant database, those that did not match a gene, and all sequence tags generated by trapping processes designed to capture introns rather than exons.
