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The molar ellipiticity values were imported into the K2d program [ 42] which uses a database comparison to predict the % α-helix, % β-sheet, and % random coil.
Open reading frames and translation of the contigs were carried out with the ExPASy translate tool [ 10] and confirmed by BLASTx with the GenBank NR database comparison to related sequences, as well as by comparison among the T. aestivum caleosin sequences as the data set was developed.
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TargetScan was used to predict the gene targets of DE miRNAs, because it is the most advanced, respected, widely used and relatively conservative database in comparison to other databases (Additional file 4).
Our goals were to evaluate an oncology EMR database in comparison to a large cancer registry and two claims databases to characterize differences between the databases, and to subsequently use these comparisons to help in estimating characteristics in a broader target cancer population in the US.
As a consequence, we expect the absolute overhead to potentially more significant (i.e. a higher relative overhead) for low-latency setups (e.g. a single node setup or an in-memory database), in comparison to setups featuring more network latency or disk I/O (e.g. a database cluster or a disk-intensive setup).
The greater difference in the number of high confidence peptides versus proteins detected using the custom database in comparison to the MSDB indicates that the most important impact of implementing the custom database was that it afforded the identification of more high confidence peptides per protein than could be achieved using the MSDB.
Furthermore, our encoding is a model-based encoding that reduces the memory usage in a database in comparison to the storage of multiple conformers of a molecule.
The taxadiene was analyzed according to NIST98 and WILEY7.0 mass spectrum database, by comparison to a nonadecane (Sigma) internal standard (1 μg/mL), to estimate total taxadiene content.
Isolates positive for any resistance determinant or int gene were further studied by extraction of the relevant Velvet contig from the BIGS database and comparison to the genomes of the pneumococcal reference strains R6 and PMEN1 (Genbank accession no. AE007317.1 and NC_011900.1, respectively) using the Artemis Comparison Tool (ACT) [ 45].
In such cases, rigorous guidelines for the inclusion of metadata are crucial, as these metadata are a necessary prerequisite for the (automated or manual) selection of relevant, trusted data from the database for comparison to new datasets or inclusion in data-mining investigations.
Further all products were purified and subjected to di-deoxy sequencing at the Clemson University Genomics Institute and database comparisons made to verify identity of PCR products.
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