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Furthermore, a second databank search to update survival and adverse event information for final revision was performed in September 2014.
In view of the rather broad specificity of the frog isomerase, we made a databank search for potential substrates of such an enzyme.
A databank search of the amplified sequences revealed 99% and 100% homology with the ITS region from Kluyveromyces marxianus which represents the teleomorph form of Candida kefyr.
Peak lists generated for individual bands from the same gel lane were merged together into a single file before databank search submission.
Using a consensus sequence derived from these results, a databank search revealed that many peptides isolated from skin secretions of different frogs would be potential substrates for such an enzyme.
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For that, appropriate primers were designed based on extensive literature and databank searches.
New computational tools are being developed in several different areas, ranging from better algorithms for protein identification and measurements of statistical confidence in identification, to de novo peptide sequencing and whole genome databank searches.
Data processing and databank searching were performed with Mascot software (Matrix Science, Beachwood, OH) and a database containing forward and decoy sequences (RSUE database) that was constructed using the USA300_FPR3757 sequence.
Data processing and databank searching were performed with Mascot software (Matrix Science).
Data processing and databank searching were performed with Mascot software version 2.1 (Matrix Science, Beachwood, OH).
Databank searches were carried out by using BLAST [ 44] and the KEGG (Kyoto Encyclopedia of Genes and Genomes; http://www.genome.ad.jp/) database [ 45].
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