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Data were scored twice independently to ensure minimal scoring errors; there were a number of loci that were out of HWE with a heterozygote deficiency for both jack pine and lodgepole pine (Table 2).
Autocorrelation data were scored to quantify the periodicity using a newly developed scoring system.
Microsatellite data were scored in GENEMAPPER v.3.7 (Applied Biosystems) and sequence data were assembled and manually checked in GENEIOUS v.4.7.6 (Biomatters, Auckland, New Zealand).
The time-series data were scored as circadian only when both the manual and MESA analyses detected the rhythms with a period between 18 and 36 hr.
The data were scored by an experienced polygraph examiner who was unaware of subject assignment to conditions and with a computer algorithm known as the Objective Scoring System Version 2 (OSS2).
Data were scored after 75 and 90 days of culture.
Patient data were scored for post-operative complications.
Since AFLPs are dominant markers, data were scored as binary data.
The following demographic and clinical data were scored: sex, age, fracture type, affected side and type of hospital.
Data were scored, binned, and subsequently re-scored to check for errors.
Following the second round of assays, all round two data were scored on this locked model.
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