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The selenomethionine and native BASS data were refined using PHENIX program to Rfree/ Rwork (0.27/0.24) and 0.25/0.22, respectively.

The raw data were refined using two filtering steps: (1) Contaminant filtering: adapter sequences may be introduced into raw reads during the library construction process.

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The resulting data was refined using a score value of 7 to match the approximate lowest score obtained in the predicted TFBSs data file at 95% threshold.

Laboratory values in this data set were refined using limits as follows: albumin, 1.0 5.0 g/dL; BG, 30 500 mg/dL; Hb, 3 20 g/dL; and HbA1c 1–12 mg/dL, respectively.

Crystals of H83A HasAp were obtained from three different conditions (see Experimental Procedures), and the corresponding structures were refined using data diffracting to 0.89 Å (H83Aortho), 1.32 Å (H83Amono), and 1.25 Å (H83ApH5.4) resolution.

Some gene models were refined using EST data (Additional file 2).

Two independent structures of the post-strand transfer states, STCMn* (early intermediate) and STCMn (late), were refined using diffraction data collected from TCC crystals incubated in the presence of Mn2+ for 2.5 min and 2 h, respectively.

The htt protein sequences of vertebrates extracted from Swiss-Prot, TrEMBL and REFSEQ databases (see Additional file 5) were refined using genomic data, and similarity criteria to experimentally well-characterized huntingtin proteins.

The component models were refined using actual operation data, resulting in precise simulation of the reference operation without leakage.

A total of 369 parameters were refined using no restraints and 2 data.

Thanks to recent techniques such as array-CGH (Shaw- Smith et al., 2004; Vermeesch et al., 2007; Vissers et al., 2005), available localization data can be refined using experimental data.

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