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Plates were immediately read using the SECTOR Imager 6000, and data were quantitated using Discovery Workbench and SOFTmax PRO 4.0 software.
Data were quantitated using Agilent Chemstation and internal standards.
Data were quantitated using an imager, ChemiDocTM MP with Imagelab Version 4.0.1 software.
Data were quantitated using ImageQuant (Amersham Biosciences), and a binding site was considered to be regulated only if it showed reciprocal regulation in the overexpression and the shRNA cell lines.
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Live and dead cells were quantitated using flow cytometry (FACScan, BD Biosciences), and data were analyzed using CELLQuestPro Version 3.3 (BD Biosciences).
Arrays were quantitated using GenePix (http://www.moleculardevices.com).
Protein concentrations were quantitated using the Bradford assay (BioRad).
Bands were quantitated using the integrated density function of ImageJ.
Proteins were quantitated using the 2D Quant Kit (GE Healthcare).
Relative intensities were quantitated using MultiGauge software (Fujifilm).
Spots were quantitated using an AID ELISPOT High Resolution Reader System Cell Technology , Inc, Columbia, MD).
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CEO of Professional Science Editing for Scientists @ prosciediting.com