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Data were quantified with Leica LAS AF (version 1.9.0; Leica).
Data were quantified with Analyst 1.4.2 software (Applied Biosystems, Foster City, CA).
Data were quantified with Analyst 1.4.2 software (Applied Biosystems, Foster City, CA) and finally exported for statistical analysis.
Data were quantified with the Living Imaging software by using absolute photon counts (photons/s) in an ROI, manually drawn to outline the BLI signal of the brain.
Immunoblot data were quantified with ImageLab software (BioRad).
Fluorescence data were quantified with Amira 5.4 software (VSG) (Burlington, MA, USA).
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Following reconstruction, image data was quantified with Hermes BRASS [11] and Southampton [12] methods.
Protein expression data was quantified with Quantity One Software (Bio-Rad).
Demographic data were quantified and analysed with non parametric statistical tests (Chi Squared test) with a P value of 0.05 or less than being considered significant.
Data were quantified blinded to treatment with Living Image software (Perkin-Elmer, Akron, OH, USA) according to defined regions of interest encompassing the lesion and background.
Data were quantified using a 5-point [0-1000 ppb (ng/g)] calibration curve with external standards.
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