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Data were quantified as mean number of neurons/section±s.e.m.m
Data were quantified as arbitrary densitometry intensity units by Gelpro32 software package (Version 3.1, Media Cybernetics, Marlow, UK).
ISBN 3 900051- 07-0, URL http://www.R-project.org).. Gene expression data were quantified as described by the manufacturer and log2-transformed to obtain normally distributed values.
The data were quantified as described below and statistically analysed.
Data were quantified as kilocalories per kilogram of body weight expended per day (kcal/kg/day).
Data were quantified as average percentage of Cux1/total per bin and differences were analyzed using an unpaired t-test.
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In vitro uridylation data are quantified as described in (Lim et al, 2014).
Proteins were quantified as above.
RNA expression data were quantified according to the delta CT method as described (Livak & Schmittgen, 2001) and normalized to levels of TATA-box-binding protein RNA TBPP) for mouse tissue and cultured cells and to 18S-rRNA for human patient samples.
Data were quantified in Fig. 10b.
Bimodality of data was quantified in the range 0 1, as (z x c)/(a x b), where z and c are the number of cases of complete sympatry and allopatry, and a and b are the number of cases of complete sympatry and allopatry that would occur if all the data were split evenly between these states [ 15].
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