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Genotype consistent HLA haplotypes for the BMDR data were constructed with the PHASE software [16].
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The data is constructed with noise that mimics the sorts of fluctuations expected in practice.
Three subsets of data were constructed: the first with 155 PN AFLPs (with 145 markers falling within the expected distribution at P < 0·05 and 10 with a significantly lower FST than expected) and the second and the third with markers putatively under selection at P < 0·05 (PS0·05) and P < 0·01 (PS0·01), consisting of 95 and 76 AFLPs, respectively.
Neighbour-joining phylogenetic trees of amino acid sequence data sets were constructed with MEGA5 (Tamura et al., 2011).
Trees using ITS data matrix were constructed with Biomphalaria helophila as outgroup since no ITS sequence of I. exustus was available.
In the case of interpopulation diversity, data matrices were constructed (with unique haplotypes) for individual genes separately, and then for all combined genes.
The data were normalised for the maximal fluorescence intensity of each compound, and expressed as percentage fluorescence intensity (% f.i .. From these field-by-field data, scattergrams were constructed with axes representing the % f.i. of each compound.
To visualize incongruence among data, split networks were constructed with the program SplitsTree4 (Huson and Bryant 2006, version 4.13.1) under default settings using the 137,941 polymorphic sites in the core genome.
Graphical data displays given here were constructed with R version 2.15.0 (R Foundation for Statistical Computing, Vienna, Austria).
The maps of electrojets were constructed with data from the magnetometer chains located on the Yakutsk (130° E) and Norilsk (90° E) meridians.
The effect of the filtering is illustrated in Additional file 2. Hence, three matrices were constructed with data for 1066 Ensembl gene IDs in 23 cell lines that were present in the datasets obtained from all three platforms.
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